| dc.identifier.uri | http://hdl.handle.net/1951/59766 | |
| dc.identifier.uri | http://hdl.handle.net/11401/71324 | |
| dc.description.sponsorship | This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. | en_US |
| dc.format | Monograph | |
| dc.format.medium | Electronic Resource | en_US |
| dc.language.iso | en_US | |
| dc.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dc.type | Dissertation | |
| dcterms.abstract | Biofilms, multi-cellular sessile communities of bacteria, are known to account for bacterial persistence and antibiotic resistance. Nitric oxide (NO) has been shown to induce biofilm dispersal at sub-lethal concentrations in many species. For example, in the cystic-fibrosis associated bacterium, Pseudomonas aeruginosa, NO is reported to regulate biofilm dispersal through cyclic di-GMP signaling. In this thesis work, I demonstrate that Swoo_2750 from Shewanella woodyi encodes a Heme-Nitric oxide/OXygen binding domain (H-NOX), a protein that binds NO with approximately picomolar sensitivity. I demonstrate further that SwH-NOX is co-cistronic and directly interacts with Swoo_2751, a bi-functional diguanylate cyclase (DGC), which exhibits both c-di-GMP synthesis and hydrolysis activities. Through steady-state kinetic analyses, I conclude that NO bound H-NOX interacts with DGC and induces a 15-fold increase in c-di-GMP hydrolysis as well as a 90% decrease in c-di-GMP synthesis compared to the effect on DGC found with unligated H-NOX. I have correlated these biochemical data with measurements of in vivo c-di-GMP concentrations and assessments of biofilm formation, both in wildtype and a mutant (Δhnox) strain of S. woodyi. These studies lead to the conclusion that this H-NOX signaling pathway provides a molecular-level explanation for the rapid dispersal of biofilms that has been observed in the presence of NO. Finally, I have explored the source of NO used in H-NOX signaling, especially the role of anaerobic respiration in regulation of biofilm formation in S. woodyi. In summary, I present here my effort towards understanding nitric oxide signaling in biofilm regulation. | |
| dcterms.available | 2013-05-22T17:35:07Z | |
| dcterms.available | 2015-04-24T14:47:02Z | |
| dcterms.contributor | Boon, Elizabeth M | en_US |
| dcterms.contributor | Sampson, Nicole S | en_US |
| dcterms.contributor | Seeliger, Jessica C | en_US |
| dcterms.contributor | Johnson, Roger A. | en_US |
| dcterms.creator | Liu, Niu | |
| dcterms.dateAccepted | 2013-05-22T17:35:07Z | |
| dcterms.dateAccepted | 2015-04-24T14:47:02Z | |
| dcterms.dateSubmitted | 2013-05-22T17:35:07Z | |
| dcterms.dateSubmitted | 2015-04-24T14:47:02Z | |
| dcterms.description | Department of Chemistry | en_US |
| dcterms.extent | 117 pg. | en_US |
| dcterms.format | Monograph | |
| dcterms.format | Application/PDF | en_US |
| dcterms.identifier | Liu_grad.sunysb_0771E_11118 | en_US |
| dcterms.identifier | http://hdl.handle.net/1951/59766 | |
| dcterms.identifier | http://hdl.handle.net/11401/71324 | |
| dcterms.issued | 2012-08-01 | |
| dcterms.language | en_US | |
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Previous issue date: 1 | en |
| dcterms.provenance | Made available in DSpace on 2015-04-24T14:47:02Z (GMT). No. of bitstreams: 3
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Previous issue date: 1 | en |
| dcterms.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dcterms.subject | Chemistry--Biochemistry | |
| dcterms.subject | biofilm, c-di-GMP, diguanylate cyclase, nitric oxide, phosphodiesterase | |
| dcterms.title | Nitric oxide regulation of c-di-GMP metabolism and biofilm formation in Shewanella woodyi | |
| dcterms.type | Dissertation | |
