| dc.identifier.uri | http://hdl.handle.net/1951/60292 | |
| dc.identifier.uri | http://hdl.handle.net/11401/71549 | |
| dc.description.sponsorship | This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. | en_US |
| dc.format | Monograph | |
| dc.format.medium | Electronic Resource | en_US |
| dc.language.iso | en_US | |
| dc.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dc.type | Dissertation | |
| dcterms.abstract | The expression of the sloppy-paired 1 (slp1) gene in the gastrula stage Drosophila embryo is controlled by the interplay of four transcription factors Runt, Even-skipped (Eve), Fushi-tarazu (Ftz), and Odd-paired (Opa) with two distinct cis-regulatory enhancer elements, the distal early stripe element (DESE) and the proximal early stripe element (PESE). The stripe pattern of slp1 is the result of non-additive interactions between these two enhancers and is context-dependent. Using chromatin immunoprecipitation (ChIP) to examine a number of reporter constructs, I found DESE mediates Runt dependent activation by facilitating pre-initiation complex formation on the slp1 promoter. This DESE-dependent activation is influenced by the extent of promoter- proximal DNA upstream of the transcription start site and involves a mechanism that induces nucleosome depletion around the promoter. This effect is specifically important for DESE activation but not PESE activation. ChIP experiments comparing wild-type versus repressed states of DESE-lacZ and PESE-lacZ reporter genes indicate that Eve represses PESE-lacZ expression by blocking the elongation step of the transcription cycle. This repression involves the regulated association of the elongation factor P-TEFb and phosphorylation of Ser2 in the C-terminal domain of RNA polymerase II. Runt and Ftz repress both DESE and PESE. Interestingly, Runt and Ftz repress DESE-lacZ by the same mechanism as Eve dependent repression of PESE, that is by inhibition of transcription elongation. However, Runt and Ftz repress PESE-lacZ by blocking transcription initiation. Analysis of the conserved domains of Runt revealed that C-terminal domain of Runt (region VIII) is involved in the repression of both DESE and PESE, and that this region is not required for activation of DESE. | |
| dcterms.available | 2013-05-24T16:38:22Z | |
| dcterms.available | 2015-04-24T14:47:52Z | |
| dcterms.contributor | Thomsen, Gerald H | en_US |
| dcterms.contributor | Gergen, J Peter | en_US |
| dcterms.contributor | Sternglanz, Rolf | en_US |
| dcterms.contributor | Haltiwanger, Robert S | en_US |
| dcterms.contributor | Kernan, Maurice | en_US |
| dcterms.creator | Hang, Saiyu | |
| dcterms.dateAccepted | 2013-05-24T16:38:22Z | |
| dcterms.dateAccepted | 2015-04-24T14:47:52Z | |
| dcterms.dateSubmitted | 2013-05-24T16:38:22Z | |
| dcterms.dateSubmitted | 2015-04-24T14:47:52Z | |
| dcterms.description | Department of Biochemistry and Structural Biology | en_US |
| dcterms.extent | 97 pg. | en_US |
| dcterms.format | Monograph | |
| dcterms.format | Application/PDF | en_US |
| dcterms.identifier | http://hdl.handle.net/1951/60292 | |
| dcterms.identifier | http://hdl.handle.net/11401/71549 | |
| dcterms.issued | 2012-08-01 | |
| dcterms.language | en_US | |
| dcterms.provenance | Made available in DSpace on 2013-05-24T16:38:22Z (GMT). No. of bitstreams: 1
StonyBrookUniversityETDPageEmbargo_20130517082608_116839.pdf: 41286 bytes, checksum: 425a156df10bbe213bfdf4d175026e82 (MD5)
Previous issue date: 1 | en |
| dcterms.provenance | Made available in DSpace on 2015-04-24T14:47:52Z (GMT). No. of bitstreams: 3
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StonyBrookUniversityETDPageEmbargo_20130517082608_116839.pdf: 41286 bytes, checksum: 425a156df10bbe213bfdf4d175026e82 (MD5)
Previous issue date: 1 | en |
| dcterms.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dcterms.subject | development, Drosophila, enhancer, sloppy-paired 1, transcription, transcription factor | |
| dcterms.subject | Developmental biology--Molecular biology--Genetics | |
| dcterms.title | Cis-regulatory contributions to the regulation of sloppy-paired 1 transcription initiation and elongation | |
| dcterms.type | Dissertation | |
