| dc.identifier.uri | http://hdl.handle.net/1951/55602 | |
| dc.identifier.uri | http://hdl.handle.net/11401/72651 | |
| dc.description.sponsorship | This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. | en_US |
| dc.format | Monograph | |
| dc.format.medium | Electronic Resource | en_US |
| dc.language.iso | en_US | |
| dc.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dc.type | Dissertation | |
| dcterms.abstract | Production and breakdown of certain phosphoinositides plays key roles in a variety of cellular processes. Among these, phosphatidylinositol-4, 5-phosphate (PIP <sub>2</sub>) is well known for its critical functions in membrane trafficking, cytoskeletal organization, and signal transduction. Type I phosphatidylinositol-4-phosphate 5-kinase (PIPKI) is the main enzyme responsible for the synthesis of PIP<sub>2</sub>. Although PIPKI activity is regulated by small G proteins and phosphatidic acid (PA), the contribution of these upstream regulators in actin cytoskeletal reorganization remains unclear. The binding region of some other PA-regulated proteins has been identified. From these, it appears that binding may either be direct or electrostatic in nature. I have identified basic residues in the proposed membrane-binding region of PIPKIΓ that are required for membrane translocation, actin reorganization, and stimulation by PA. I also demonstrate here that the direct binding of PIPKI to PA through these residues is required for these important functions. | |
| dcterms.available | 2012-05-15T18:06:22Z | |
| dcterms.available | 2015-04-24T14:53:02Z | |
| dcterms.contributor | Joav Prives | en_US |
| dcterms.contributor | Du, Guangwei | en_US |
| dcterms.contributor | Deborah A. Brown | en_US |
| dcterms.contributor | Michael A. Frohman. | en_US |
| dcterms.creator | Roach, Akua Ntiamoah | |
| dcterms.dateAccepted | 2012-05-15T18:06:22Z | |
| dcterms.dateAccepted | 2015-04-24T14:53:02Z | |
| dcterms.dateSubmitted | 2012-05-15T18:06:22Z | |
| dcterms.dateSubmitted | 2015-04-24T14:53:02Z | |
| dcterms.description | Department of Molecular and Cellular Pharmacology | en_US |
| dcterms.format | Monograph | |
| dcterms.format | Application/PDF | en_US |
| dcterms.identifier | http://hdl.handle.net/1951/55602 | |
| dcterms.identifier | Roach_grad.sunysb_0771E_10274.pdf | en_US |
| dcterms.identifier | http://hdl.handle.net/11401/72651 | |
| dcterms.issued | 2010-08-01 | |
| dcterms.language | en_US | |
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Previous issue date: 1 | en |
| dcterms.provenance | Made available in DSpace on 2015-04-24T14:53:02Z (GMT). No. of bitstreams: 3
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Previous issue date: 1 | en |
| dcterms.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dcterms.subject | actin reorganization, Lipid Signaling, phosphatidic acid, PIPKI | |
| dcterms.subject | Health Sciences, Pharmacology | |
| dcterms.title | The Role of Phosphatidic Acid in PIPKI-regulated Actin Reorganization | |
| dcterms.type | Dissertation | |
