| dc.identifier.uri | http://hdl.handle.net/11401/76153 | |
| dc.description.sponsorship | This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. | en_US |
| dc.format | Monograph | |
| dc.format.medium | Electronic Resource | en_US |
| dc.language.iso | en_US | |
| dc.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dc.type | Dissertation | |
| dcterms.abstract | The work in this thesis provides mulit-scale contributions towards understanding the effects of trace metals on the pathological mineralization process relating to both the development of healthy bone tissue, the diseased state of osteoporosis, and microcalcifications which develop in breast cancers. A protein level study was performed on ECM protein fibronectin, which plays a role in cell adhesion. The protein studies showed zinc interactions with fibronectin and its fragment, anastellin, to influence protein structure. Zinc is also shown to decrease cell migration in vitro, which may be influenced by changes in fibronectin ECM structure. The effects of osteoporosis on micronutrient composition in vivo were examined using the technique of x-ray fluorescence (XRF) in an ovariectomized rat model. Compared to healthy bone, subtle difference are observed in zinc and iron in osteoporotic rat bones, showing micronutrients may play an important role in healthy bone regulation. Effects of micronutrient zinc was used to inhibit microcalcification formation in breast cancers. Microcalcifications have been linked malignancy of breast cancers, but the process of microcalcification formation has yet to be well understood. In this work, exogenous zinc is used to inhibit microcalcification formation, and metastatic potential in both a 2D and 3D spheroid environment. A novel in vitro self-assembled three dimensional multi-cellular tumor spheroid (MCTS) model for the study of breast cancer microcalcifications was developed for this experiment. A MCTS model for studying breast cancer microcalcifications has potential to be used in drug discovery, or for basic research applications studying mechanisms of microcalcification formation, which are still not fully understood. Taken together this study uses a multi-scale approach to gain a better understanding of micronutrients involved in pathological mineralization. | |
| dcterms.available | 2017-09-20T16:42:30Z | |
| dcterms.contributor | Meng, Yizhi | en_US |
| dcterms.contributor | Venkatesh, T. A. | en_US |
| dcterms.contributor | Kim, Tae Jin | en_US |
| dcterms.contributor | Yin, Wei. | en_US |
| dcterms.creator | Rammelkamp, Derek | |
| dcterms.dateAccepted | 2017-09-20T16:42:30Z | |
| dcterms.dateSubmitted | 2017-09-20T16:42:30Z | |
| dcterms.description | Department of Materials Science and Engineering | en_US |
| dcterms.extent | 222 pg. | en_US |
| dcterms.format | Application/PDF | en_US |
| dcterms.format | Monograph | |
| dcterms.identifier | http://hdl.handle.net/11401/76153 | |
| dcterms.issued | 2017-05-01 | |
| dcterms.language | en_US | |
| dcterms.provenance | Made available in DSpace on 2017-09-20T16:42:30Z (GMT). No. of bitstreams: 1
Rammelkamp_grad.sunysb_0771E_13273.pdf: 6546997 bytes, checksum: b596db565fd99bad863420cc98e00927 (MD5)
Previous issue date: 1 | en |
| dcterms.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dcterms.subject | Materials Science -- Chemical engineering | |
| dcterms.title | A Multi-Scale Study on the Role of Trace Metals on Physiological and Pathological | |
| dcterms.type | Dissertation | |
