Candida albicans Adenylyl cyclase – cAMP Pathway and Hyphal Growth
Candida albicans Adenylyl cyclase – cAMP Pathway and Hyphal Growth
| dc.identifier.uri | http://hdl.handle.net/11401/76480 | |
| dc.description.sponsorship | This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. | en_US |
| dc.format | Monograph | |
| dc.format.medium | Electronic Resource | en_US |
| dc.language.iso | en_US | |
| dc.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dc.type | Dissertation | |
| dcterms.abstract | The human opportunistic fungal pathogen Candida albicans can undergo a transition from budding to hyphal growth in response to host cues that promotes tissue invasion and dissemination through the host. Accompanying the morphogenic change is an upregulation of genes that promote virulence. Morphogenic switching and subsequent gene regulation are required for full virulence in the host and are an area of intense study. It has been shown that increased production of cAMP by Cyr1 Adenylyl cyclase can induce hyphal growth. Further, it was proposed that activation of Cyr1 and an increase in cAMP were necessary for all hyphal growth in C. albicans. The main evidence for this necessity is that cells lacking Adenylyl cyclase (cyr1Δ) fail to induce hyphal growth in response to most physiological inducers. This evidence is not conclusive as deletion of CYR1 severely affects cellular health. I have demonstrated that signaling through Cyr1 and cAMP are not required for hyphal morphogenesis or virulence gene induction. I propose that cAMP-independent pathways are responsible for much of the morphogenic and gene regulatory signals produced by physiological inducers. My findings shed light on hyphal signaling in Candida albicans and will aid in the identification of drug targets and the development of novel therapeutics. | |
| dcterms.abstract | The human opportunistic fungal pathogen Candida albicans can undergo a transition from budding to hyphal growth in response to host cues that promotes tissue invasion and dissemination through the host. Accompanying the morphogenic change is an upregulation of genes that promote virulence. Morphogenic switching and subsequent gene regulation are required for full virulence in the host and are an area of intense study. It has been shown that increased production of cAMP by Cyr1 Adenylyl cyclase can induce hyphal growth. Further, it was proposed that activation of Cyr1 and an increase in cAMP were necessary for all hyphal growth in C. albicans. The main evidence for this necessity is that cells lacking Adenylyl cyclase (cyr1Δ) fail to induce hyphal growth in response to most physiological inducers. This evidence is not conclusive as deletion of CYR1 severely affects cellular health. I have demonstrated that signaling through Cyr1 and cAMP are not required for hyphal morphogenesis or virulence gene induction. I propose that cAMP-independent pathways are responsible for much of the morphogenic and gene regulatory signals produced by physiological inducers. My findings shed light on hyphal signaling in Candida albicans and will aid in the identification of drug targets and the development of novel therapeutics. | |
| dcterms.available | 2017-09-20T16:50:23Z | |
| dcterms.contributor | Konopka, James B | en_US |
| dcterms.contributor | van der Velden, Adrianus | en_US |
| dcterms.contributor | Bliska, James. | en_US |
| dcterms.contributor | Neiman, Aaron | en_US |
| dcterms.creator | Parrino, Salvatore Michael | |
| dcterms.dateAccepted | 2017-09-20T16:50:23Z | |
| dcterms.dateSubmitted | 2017-09-20T16:50:23Z | |
| dcterms.description | Department of Molecular and Cellular Biology. | en_US |
| dcterms.extent | 80 pg. | en_US |
| dcterms.format | Application/PDF | en_US |
| dcterms.format | Monograph | |
| dcterms.identifier | http://hdl.handle.net/11401/76480 | |
| dcterms.issued | 2015-05-01 | |
| dcterms.language | en_US | |
| dcterms.provenance | Made available in DSpace on 2017-09-20T16:50:23Z (GMT). No. of bitstreams: 1 Parrino_grad.sunysb_0771E_12511.pdf: 22960487 bytes, checksum: 6a75225e3c94d5addccd37eb03a2379e (MD5) Previous issue date: 2015 | en |
| dcterms.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dcterms.subject | Adenylyl cyclase, cAMP, Candida albicans, hyphal growth, hyphal induction, pseudorevertants | |
| dcterms.subject | Molecular biology | |
| dcterms.title | Candida albicans Adenylyl cyclase – cAMP Pathway and Hyphal Growth | |
| dcterms.title | Candida albicans Adenylyl cyclase – cAMP Pathway and Hyphal Growth | |
| dcterms.type | Dissertation |
