| dc.identifier.uri | http://hdl.handle.net/11401/76890 | |
| dc.description.sponsorship | This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. | en_US |
| dc.format | Monograph | |
| dc.format.medium | Electronic Resource | en_US |
| dc.language.iso | en_US | |
| dc.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dc.type | Thesis | |
| dcterms.abstract | Homologous Recombination (HR) is an essential mechanism for double strand break (DSB) repair that generates crossovers (COs). In meiosis, HR is required for proper chromosome segregation at the first meiotic divison. The Sgs1 helicase promotes the formation of non-crossovers (NCOs) through a process called synthesis-dependent strand annealing. A set of meiosis specific genes called the ZMM genes, protects strand invasion intermediates from Sgs1, resulting in the formation of a specific class of COs that are distributed throughout the genome. The transverse filament protein, Zip1 is encoded by one of the ZMM genes, and phosphorylation of Zip1 on four adjacent serines in its C terminus are required for the ZMM pathway of recombination. Previous work has shown the absence of SGS1 combined with a nonphosphorylatable version of ZIP1, zip1-4A, triggers the meiotic recombination checkpoint, resulting in meiotic prophase arrest. Srs2 is a less well studied helicase in meiosis, which like Sgs1, exhibits an anti-recombinase function during DSB repair in vegetative cells. The goal of my thesis was to see whether depletion of SRS2 has a similar genetic interaction in zip1-4A, which would suggest a role for SRS2 during meiosis similar to SGS1. | |
| dcterms.available | 2017-09-20T16:51:23Z | |
| dcterms.contributor | Hollingsworth, Nancy M | en_US |
| dcterms.contributor | Luk, Ed. | en_US |
| dcterms.creator | Joseph, Dimitri | |
| dcterms.dateAccepted | 2017-09-20T16:51:23Z | |
| dcterms.dateSubmitted | 2017-09-20T16:51:23Z | |
| dcterms.description | Department of Biochemistry and Cell Biology | en_US |
| dcterms.extent | 53 pg. | en_US |
| dcterms.format | Monograph | |
| dcterms.format | Application/PDF | en_US |
| dcterms.identifier | http://hdl.handle.net/11401/76890 | |
| dcterms.issued | 2017-05-01 | |
| dcterms.language | en_US | |
| dcterms.provenance | Made available in DSpace on 2017-09-20T16:51:23Z (GMT). No. of bitstreams: 1
Joseph_grad.sunysb_0771M_13252.pdf: 1853139 bytes, checksum: 436434db1ffaede2cebf3d6cf405b3a3 (MD5)
Previous issue date: 1 | en |
| dcterms.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dcterms.subject | homologous recombination, srs2, zip1 | |
| dcterms.subject | Cellular biology | |
| dcterms.title | Characterization of a genetic interaction between the Srs2 helicase and phosphorylated Zip1 during budding yeast meiosis | |
| dcterms.type | Thesis | |
