| dc.identifier.uri | http://hdl.handle.net/11401/76892 | |
| dc.description.sponsorship | This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. | en_US |
| dc.format | Monograph | |
| dc.format.medium | Electronic Resource | en_US |
| dc.language.iso | en_US | |
| dc.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dc.type | Thesis | |
| dcterms.abstract | The Kin28 subunit of the general transcription factor complex, TFIIH, is a kinase that phosphorylates the heptapeptide repeats at serine position 5 (Ser5) on the C-terminal domain of RNA polymerase II (Pol II). Optimal Ser5 phosphorylation is important during transcription for Pol II to transition from the initiation step to the elongation step in a process called promoter escape. During transcription initiation, the nucleosome directly downstream of the promoter, called +1, must be disassembled before Pol II can engage the transcription start site. Previous work showed that the transcription machinery plays a direct role in the disassembly of the +1 nucleosome, but the exact mechanism is currently unknown. We hypothesize that the disassembly of the +1 nucleosome occurs during promoter escape. To test this idea, I attempted to conditionally deplete Kin28, in the yeast, Saccharomyces cerevisiae, to block promoter escape. I then applied a modified genomic approach to map nascent Pol II-associated RNA molecules before and after Kin28 depletion. The Kin28 depletion failed to block promoter escape, possibly due to incomplete removal of Kin28 from the nucleus. Instead, I found that the Kin28 depleted strain accumulated Pol II-associated read-through transcripts beyond the transcription termination site suggesting that Kin28 plays a role in the processing of the nascent RNA of protein-coding genes. | |
| dcterms.available | 2017-09-20T16:51:23Z | |
| dcterms.contributor | Luk, Ed | en_US |
| dcterms.contributor | Hollingsworth, Nancy M | en_US |
| dcterms.creator | Labuz, Daniel Roy | |
| dcterms.dateAccepted | 2017-09-20T16:51:23Z | |
| dcterms.dateSubmitted | 2017-09-20T16:51:23Z | |
| dcterms.description | Department of Biochemistry and Cell Biology | en_US |
| dcterms.extent | 49 pg. | en_US |
| dcterms.format | Application/PDF | en_US |
| dcterms.format | Monograph | |
| dcterms.identifier | http://hdl.handle.net/11401/76892 | |
| dcterms.issued | 2016-12-01 | |
| dcterms.language | en_US | |
| dcterms.provenance | Made available in DSpace on 2017-09-20T16:51:23Z (GMT). No. of bitstreams: 1
Labuz_grad.sunysb_0771M_13119.pdf: 3574928 bytes, checksum: 84a7cd55029d3655d59b938d7d5602c2 (MD5)
Previous issue date: 1 | en |
| dcterms.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dcterms.subject | Biochemistry -- Molecular biology | |
| dcterms.title | The role of the Kin28 protein kinase in chromatin remodeling and transcription termination | |
| dcterms.type | Thesis | |
