| dc.identifier.uri | http://hdl.handle.net/11401/76909 | |
| dc.description.sponsorship | This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. | en_US |
| dc.format | Monograph | |
| dc.format.medium | Electronic Resource | en_US |
| dc.language.iso | en_US | |
| dc.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dc.type | Thesis | |
| dcterms.abstract | Microglia are the resident immune cells of the central nervous system (CNS). They are involved in the maintenance of brain homeostasis not only as activated rapid responders to pathological changes in the CNS, but also when non-activated through continually extending and retracting of processes to survey the brain parenchyma and make contacts with neuronal synapses. Recent work has shown that microglia regulate the number of presynaptic terminals and glutamate receptor expression. Elimination of microglia in hippocampal brain slices results in an increase in the frequency of excitatory postsynaptic current (EPSC), and replenishment of microglia restores the EPSC frequency to normal levels. To asses if these changes in synaptic physiology with and without microglia have an effect on behavior we inject clodronate or PBS into the CA1 hippocampus of mice. Clodronate is internalized by macrophages and causes their selective apoptosis. Behavioral studies revealed that, compared to vehicle-injected mice, clodronate-injected mice exhibited a decrease in artificially induced grooming behavior and rate of spatial learning. Clodronate-injected mice did not exhibit any difference in activity level or rearings. Together with the previous data, our findings demonstrate that the non-activated microglia in the hippocampus modulate synaptic activity by regulating number of functional synapses and affect animal behavior. | |
| dcterms.available | 2017-09-20T16:51:25Z | |
| dcterms.contributor | Robinson, John. | en_US |
| dcterms.contributor | Tsirka, Styliani-Anna E | en_US |
| dcterms.creator | Danver, Joan | |
| dcterms.dateAccepted | 2017-09-20T16:51:25Z | |
| dcterms.dateSubmitted | 2017-09-20T16:51:25Z | |
| dcterms.description | Department of Biochemistry and Cell Biology. | en_US |
| dcterms.extent | 32 pg. | en_US |
| dcterms.format | Application/PDF | en_US |
| dcterms.format | Monograph | |
| dcterms.identifier | http://hdl.handle.net/11401/76909 | |
| dcterms.issued | 2015-08-01 | |
| dcterms.language | en_US | |
| dcterms.provenance | Made available in DSpace on 2017-09-20T16:51:25Z (GMT). No. of bitstreams: 1
Danver_grad.sunysb_0771M_11642.pdf: 1129845 bytes, checksum: 6dcf15584cadd1fa42e49342bb1e8dd8 (MD5)
Previous issue date: 2013 | en |
| dcterms.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dcterms.subject | Pharmaceutical sciences | |
| dcterms.title | Microglia ablation in the hippocampus affects mouse behavior | |
| dcterms.type | Thesis | |
