| dc.identifier.uri | http://hdl.handle.net/11401/76921 | |
| dc.description.sponsorship | This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. | en_US |
| dc.format | Monograph | |
| dc.format.medium | Electronic Resource | en_US |
| dc.language.iso | en_US | |
| dc.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dc.type | Dissertation | |
| dcterms.abstract | In Saccharomycs cerevisiae, spores are a quiescent, stress-resistant cell type, which can survive for extended periods of time in unfavorable conditions until nutrient conditions are suitable. The outer layers of the spore wall contain chitosan and dityrosine, which are macromolecules specific for the spore. These layers are critical for resistance of the spores to adverse environmental stresses. The dityrosine layer is an unique polymer component of cross-linked N, N'-bisformyl dityrosine. This layer provides a shield from protein-sized attack such as lytic enzymes. For structural studies, we took advantage of the insolubility of the spore wall materials by using solid-state NMR to examine the components of the spore wall outer layers and the cross-linkage among these components. These studies identified a previously unknown component, which we term " Chi" . To reveal the proteins involved in dityrosine layer assembly, a synthetic genetic array approach was performed. The study uncovers a highly redundant genetic network, and also identifies new genes involved in outer spore wall formation. Several of the genes have paralogs in the yeast genome. Deletion of the paralog sets cause dityrosine layer defects. Taken together, we conclude that there are different levels of redundancy to regulate the synthesis of dityrosine layer. The first aspect of redundancy is the genes listed in the network, which may play parallel roles in the alternative pathways of dityrosine layer assembly. The other level of redundancy is the paralogs from the network, which may function on the same pathway of dityrosine layer synthesis. | |
| dcterms.available | 2017-09-20T16:51:26Z | |
| dcterms.contributor | Neiman, Aaron M | en_US |
| dcterms.contributor | Goroff, Nancy | en_US |
| dcterms.contributor | London, Erwin | en_US |
| dcterms.contributor | Smith, Steven O | en_US |
| dcterms.contributor | Sternglanz, Rolf. | en_US |
| dcterms.creator | Lin, Pei-Chen Coney | |
| dcterms.dateAccepted | 2017-09-20T16:51:26Z | |
| dcterms.dateSubmitted | 2017-09-20T16:51:26Z | |
| dcterms.description | Department of Biochemistry and Cell Biology. | en_US |
| dcterms.extent | 106 pg. | en_US |
| dcterms.format | Application/PDF | en_US |
| dcterms.format | Monograph | |
| dcterms.identifier | http://hdl.handle.net/11401/76921 | |
| dcterms.issued | 2013-12-01 | |
| dcterms.language | en_US | |
| dcterms.provenance | Made available in DSpace on 2017-09-20T16:51:26Z (GMT). No. of bitstreams: 1
Lin_grad.sunysb_0771E_11545.pdf: 14619882 bytes, checksum: 805da6be636868e2b2364828995871ff (MD5)
Previous issue date: 1 | en |
| dcterms.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dcterms.subject | Genetic redundancy, Lipid droplets, Solid state NMR, Spore wall, Sporulation, Yeast | |
| dcterms.subject | Genetics | |
| dcterms.title | Structure and Assembly of Saccharomyces cerevisiae Outer Spore Wall | |
| dcterms.type | Dissertation | |
