| dc.identifier.uri | http://hdl.handle.net/11401/76925 | |
| dc.description.sponsorship | This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. | en_US |
| dc.format | Monograph | |
| dc.format.medium | Electronic Resource | en_US |
| dc.language.iso | en_US | |
| dc.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dc.type | Thesis | |
| dcterms.abstract | Surface modification of viruses is of great interest for therapeutic gene delivery. Numerous viruses have been, and continue to be engineered in an attempt to optimize them for targeted in vivo gene therapy. Adenovirus has been shown by a myriad of publications to be the most efficient delivery vehicle in different cell types. But while it is the preferred delivery agent in many gene therapy studies, drawbacks such as off-targeting, immune response and toxicity still hinder it from being used in clinical studies. In an attempt to modify the surface of Adenovirus efficiently, our lab developed a simple two-step labeling protocol to modify Adenovirus particles with specific ligands in a time efficient manner without affecting the virus infectivity. We have shown that our method is able to produce site specific surface modified infectious Adenoviruses with high titers. For future studies, we intend to use this approach to engineer dually modified Adenovirus particles with a targeting ligand and a PET scanning radioactive moiety (18F or 89Zr) to help visualize distribution in vivo. The results from our studies will provide useful insight into the biodistribution of Adenovirus, which can eventually help contribute to the future development of next generation of Adenovirus vectors better suited for gene delivery. | |
| dcterms.available | 2017-09-20T16:51:27Z | |
| dcterms.contributor | Carrico, Isaac S | en_US |
| dcterms.contributor | Hearing, Patrick. | en_US |
| dcterms.creator | Pal, Jai Inder | |
| dcterms.dateAccepted | 2017-09-20T16:51:27Z | |
| dcterms.dateSubmitted | 2017-09-20T16:51:27Z | |
| dcterms.description | Department of Biochemistry and Cell Biology. | en_US |
| dcterms.extent | 37 pg. | en_US |
| dcterms.format | Application/PDF | en_US |
| dcterms.format | Monograph | |
| dcterms.identifier | http://hdl.handle.net/11401/76925 | |
| dcterms.issued | 2014-12-01 | |
| dcterms.language | en_US | |
| dcterms.provenance | Made available in DSpace on 2017-09-20T16:51:27Z (GMT). No. of bitstreams: 1
Pal_grad.sunysb_0771M_12206.pdf: 647815 bytes, checksum: 1c7a064de9ee32043ac7f5a4cd1b99be (MD5)
Previous issue date: 1 | en |
| dcterms.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dcterms.subject | Biochemistry | |
| dcterms.title | Surface modification of Adenovirus type 5 vectors via two-step metabolic labeling for PET imaging | |
| dcterms.type | Thesis | |
