| dc.identifier.uri | http://hdl.handle.net/11401/77001 | |
| dc.description.sponsorship | This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. | en_US |
| dc.format | Monograph | |
| dc.format.medium | Electronic Resource | en_US |
| dc.language.iso | en_US | |
| dc.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dc.type | Dissertation | |
| dcterms.abstract | The prevalence of oral cancer has been increasing at an alarming rate in recent years. Due to a limited understanding of the cause and progression of this disease, patient prognosis remain poor, and the mortality and morbidity of the disease remain significant. The need to diagnose cancerous regions early is key to patient survival. However, the high cost and invasive nature of standard pathological analysis motivates the search for an alternative real-time imaging technology that provides accurate non-invasive early diagnosis of the disease. The goal of this thesis is to develop a line-scanned dual-axis confocal (LS-DAC) microscope for high-resolution, high-contrast, high-speed imaging suitable for real-time non-invasive clinical diagnoses. The performance of the LS-DAC microscope architecture is characterized by measuring its axial and lateral response in water as well as a homogeneous scattering phantom (Intralipid). The accuracy of the results are assessed and confirmed by comparison with results from a Monte Carlo scattering simulation of the system. In addition, a parallel comparison between the LS-DAC and our existing point-scanned dual-axis confocal (PS-DAC) microscope is performed to ensure comparable imaging quality at shallow depths. Furthermore, a heterogeneous phantom has been developed and a deconvolution algorithm utilized to aid the characterization and contrast enhancement of the DAC setup, respectively. Finally, the performance of the LS-DAC is demonstrated by imaging ex vivo and in vivo biological samples in fluorescence mode. The tabletop LS-DAC developed in this thesis research serves as a basic prototype and benchmark system for a miniaturized portable LS-DAC that is also being developed in our lab for affordable non-invasive, real-time diagnostic purposes in patients. | |
| dcterms.available | 2017-09-20T16:51:38Z | |
| dcterms.contributor | Pan, Yingtian | en_US |
| dcterms.contributor | Liu, Jonathan T.C. | en_US |
| dcterms.contributor | Entcheva, Emilia | en_US |
| dcterms.contributor | Weinacht, Thomas. | en_US |
| dcterms.creator | Wang, Danni | |
| dcterms.dateAccepted | 2017-09-20T16:51:38Z | |
| dcterms.dateSubmitted | 2017-09-20T16:51:38Z | |
| dcterms.description | Department of Biomedical Engineering. | en_US |
| dcterms.extent | 68 pg. | en_US |
| dcterms.format | Monograph | |
| dcterms.format | Application/PDF | en_US |
| dcterms.identifier | http://hdl.handle.net/11401/77001 | |
| dcterms.issued | 2014-12-01 | |
| dcterms.language | en_US | |
| dcterms.provenance | Made available in DSpace on 2017-09-20T16:51:38Z (GMT). No. of bitstreams: 1
Wang_grad.sunysb_0771E_12119.pdf: 4035834 bytes, checksum: 01b36034591ecb72708146017de6e4f9 (MD5)
Previous issue date: 1 | en |
| dcterms.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dcterms.subject | Confocal microscopy, Fluorescence imaging, In vivo imaging, Optical design, Phantom design | |
| dcterms.subject | Biomedical engineering | |
| dcterms.title | Development of a Line-scanned Dual-axis Confocal Microscope for Video-rate Diagnostic Imaging | |
| dcterms.type | Dissertation | |
