| dc.identifier.uri | http://hdl.handle.net/11401/77636 | |
| dc.description.sponsorship | This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. | en_US |
| dc.format | Monograph | |
| dc.format.medium | Electronic Resource | en_US |
| dc.language.iso | en_US | |
| dc.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dc.type | Thesis | |
| dcterms.abstract | In <italic>Saccharomyces cerevisiae</italic>, translation is regulated in meiosis <super>1</super>. Rim4 is an RNA binding protein with two RNA recognition motifs (RRMs). Previous studies <super>2</super> have shown that point mutations in either RRM knock out the translational repression of <italic>CLB3</italic>, <italic>SPO20</italic>, <italic>GIP1</italic> and <italic>SPS1</italic> in meiosis I, suggesting that the translational control of these genes may be due to Rim4. We wished to see the full range of RNAs bound by Rim4. We applied RIP-chip (RNA Immunoprecipitation/microarray) and CLIP-seq (<italic>in vivo</italic> Crosslinking and Immunoprecipitation/sequencing) to identify the RNAs co-immunoprecipitated with Rim4 in a synchronized meiosis I culture. The translationally-repressed genes co-clustering with <italic>CLB3</italic>, and translationally-repressed genes co-clustering with <italic>AMA1</italic> in ribosome footprint data <super>1</super> were enriched in Rim4 immunoprecipitates compared to the negative controls. Therefore, these two groups of translationally repressed genes seem to be the targets of Rim4. Some other genes without translational repression in meiosis I were also enriched in Rim4 immunoprecipitates. | |
| dcterms.available | 2017-09-20T16:53:07Z | |
| dcterms.contributor | Futcher, Bruce | en_US |
| dcterms.contributor | Neiman, Aaron | en_US |
| dcterms.creator | Xiao, Che | |
| dcterms.dateAccepted | 2017-09-20T16:53:07Z | |
| dcterms.dateSubmitted | 2017-09-20T16:53:07Z | |
| dcterms.description | Department of Genetics. | en_US |
| dcterms.extent | 50 pg. | en_US |
| dcterms.format | Application/PDF | en_US |
| dcterms.format | Monograph | |
| dcterms.identifier | http://hdl.handle.net/11401/77636 | |
| dcterms.issued | 2015-08-01 | |
| dcterms.language | en_US | |
| dcterms.provenance | Made available in DSpace on 2017-09-20T16:53:07Z (GMT). No. of bitstreams: 1
Xiao_grad.sunysb_0771M_11934.pdf: 1007443 bytes, checksum: 571ce46b1c653c47eaaedcf72ef32207 (MD5)
Previous issue date: 2014 | en |
| dcterms.publisher | The Graduate School, Stony Brook University: Stony Brook, NY. | |
| dcterms.subject | Biochemistry | |
| dcterms.subject | budding yeast, meiosis, Rim4, RNA binding protein, translational regulation | |
| dcterms.title | Exploring RNA Binding Pattern of Rim4 in Meiosis | |
| dcterms.type | Thesis | |
