Role of the VAN1 mannosyltransferase in Candida albicans protein glycosylation
| dc.identifier.uri | http://hdl.handle.net/11401/78226 | |
| dc.description.sponsorship | This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree. | en_US |
| dc.format | Monograph | |
| dc.format.medium | Electronic Resource | en_US |
| dc.language.iso | en_US | |
| dc.type | Thesis | |
| dcterms.abstract | The outer layer of the cell wall of Candida albicans contains glycoproteins made up of mannose-rich glycans that are critical for host-fungal interactions, including adhesion, virulence, and drug resistance. Asparagine (N)-linked glycans are added to a core oligosaccharide by mannosyltransferases in the Golgi to create the long outer chain α1,6-linked mannose “backbone” that branches with α1,2-, α1,3-, and β1,2- linked mannoses. In Saccharomyces cerevisiae, the mannosyltransferases responsible for these modifications have been well studied and characterized. Orthologs of these mannosyltransferases have been identified in C.albicans and in this study we examine VAN1, a mannosyltransferase that acts very early in elongation of the backbone. To determine the role that VAN1 plays in protein glycosylation, we used CRISPR/Cas9 to delete VAN1 in C. albicans . Phenotypic analyses demonstrated this mutation caused severe morphological, growth, and cell wall phenotypes. van1Δ mutants are defective in hyphal formation, produce increased levels of cell wall chitin, and have abnormal size and shape. Analyses of glycoprotein reporters specific for N-linked glycosylation showed loss of VAN1 resulted in severe truncation of N-linked glycans in vivo. Together, these results demonstrate the importance of VAN1 in N-glycan outer chain synthesis and the integrity of the cell wall. | |
| dcterms.available | 2018-06-21T13:38:36Z | |
| dcterms.contributor | Dean, Neta | en_US |
| dcterms.contributor | Konopka, James | en_US |
| dcterms.creator | DaSilva, Justin Gino | |
| dcterms.dateAccepted | 2018-06-21T13:38:36Z | |
| dcterms.dateSubmitted | 2018-06-21T13:38:36Z | |
| dcterms.description | Department of Biochemistry and Structural Biology | en_US |
| dcterms.extent | 50 pg. | en_US |
| dcterms.format | Application/PDF | en_US |
| dcterms.format | Monograph | |
| dcterms.identifier | http://hdl.handle.net/11401/78226 | |
| dcterms.issued | 2017-12-01 | |
| dcterms.language | en_US | |
| dcterms.provenance | Made available in DSpace on 2018-06-21T13:38:36Z (GMT). No. of bitstreams: 1 DaSilva_grad.sunysb_0771M_13575.pdf: 1173999 bytes, checksum: 2979e73205441477d055889dc6ca2936 (MD5) Previous issue date: 12 | en |
| dcterms.subject | Candida albicans | |
| dcterms.subject | Biochemistry | |
| dcterms.subject | Cell wall | |
| dcterms.subject | Cytology | |
| dcterms.subject | CRISPR/Cas9 | |
| dcterms.subject | Molecular biology | |
| dcterms.subject | Glycosylation | |
| dcterms.subject | Mannosyltransferase | |
| dcterms.subject | VAN1 | |
| dcterms.title | Role of the VAN1 mannosyltransferase in Candida albicans protein glycosylation | |
| dcterms.type | Thesis |
